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Nevertheless, during the time training course for measurement of catalytic exercise because of the apo-enzyme, the speed of substrate turnover enhanced slowly and gradually with time. The activation of the apo-enzyme was evidently as a result of binding of trace metals from the assay Alternative Considering that the activity of the apo-enzyme from the presence of five hundred μM EDTA was fewer than 2% from the exercise exhibited from the native enzyme. These final results are in line with an absolute necessity for your bound divalent cation for the expression of catalytic exercise by NagA.
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-acetyl-l-aspartate is a very good substrate for aspartoacylase from human brain (31). The kinetic parameters for your hydrolysis of 6 with NagA are shown in Table 2 This substrate is hydrolyzed at a substantially more rapidly charge than will be the corresponding N
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